Democratization and accessibility of complex engineering tools
Our goal is to make complex engineering tools in a way that is inexpensive and easy to translate to non-engineering labs. Together with collaborators from developmental biology, microbiome, infectious disease, and clinical fields, we have created multiple different tools to better study human physiology and diseases. Use the links below to inquire about more information or to collaborate.
Zonal patterning of extracellular matrix and stromal cell populations along a perfusable cellular microchannel, B Chernokal*, BJ Ferrick*, JP Gleghorn, Lab on a Chip, 2024, 24, 5238 - 5250. *indicates equal contribution
We have developed and validated a simple strategy to create an in vitro microphysiological system with regionally patterned stromal cell populations and hydrogel properties along the length of a perfused continuous tubular epithelium. This highly customizable system has wide utility for modeling epithelial and endothelial tissue interactions with heterogeneous hydrogel compositions and/or stromal cell populations to better dissect the specific microenvironmental factors that govern developmental tissue patterning.
Click here for more information or to inquire about a collaboration
Zonal Patterning Device
Modular Microphysiological Device
Democratizing a modular 2-layer microfluidic organ-on-a-chip, DJ Minahan, KM Nelson, F Ribeiro, MJ Deputy, A Zurzolo, K Byers, and JP Gleghorn, *indicates equal contribution
We have developed and validated a simple strategy to create a two layer in vitro microphysiological system within a one-time-use cell culture insert and a reusable cassette to enable fluid flow. The system enables easier cell culture than PDMS based devices and can be irreversibly sealed to enable access to the cells for assays. This highly customizable system has wide utility for modeling transport across a cell barrier and cell-cell interactions to better study physiological and pathogenic processes.
Click here for more information or to inquire about a collaboration
